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  • Purification and characterization of Anacardium occidentale (cashew) allergens Ana o 1, Ana o 2 and Ana o 3

    Jan 14th, 2016

    In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by SDS-PAGE, western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE-binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunit, both displaying micro-heterogeneity, and epitope mapping of Ana o 3 has been performed.

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